25-Hydroxy Vitamin D 
Alternate Test Names
Vitamin D3; Cholecalciferol; 25D; Vitamin D 
PLS Test Code
25D 
Laboratory
Biochemistry 
Specimen
Blood 
Analysis Performed On
Serum 
Container
Serum Separator Tube (SST) 
Container Colour
Red 
Multiple Specimens
Serum Separator Tube (SST)  
Required Volume Adult (mL)
Req Vol Paediatric (mL)
Reference Ranges
Vitamin D adequacy: >=50 nmol/L at the end of winter (level may need to be 10-20 nmol/L higher at the end of summer, to allow for seasonal decrease). Mild vitamin D deficiency: 30 - 49 nmol/L; Moderate vitamin D deficiency: 12.5 - 29 nmol/L; Severe vitami 
Turnaround Time Routine
1 Day 
Turnaround Time Urgent
 
Turnaround Other
 
Days test is performed
Daily 
Transport
Keep at room temperature 
Special Requirements
None 
Patient Preparation
None 
Collection Instructions
None 
Clinical Information
 
Sent to Reference Laboratory
No 
Ref Laboratory Name
 
Estimated Patient Fee
N/A 
Last Updated
14-Feb-2023  (Version:1.1 )

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